Effectiveness of Coleus blumei (Mayana) Leaf Crude Extract as an Alternative Staining Reagent for Urine Sediment Staining
DOI:
https://doi.org/10.65166/vdj5nq43Keywords:
Coleus blumei, Mayana leaf extract, natural stain, urine sediment, ethanol extraction, laboratory stainingAbstract
This study evaluated the effectiveness of Coleus blumei (Mayana) leaf crude extract as a potential alternative staining reagent for urine sediment staining. The study was conducted as an experimental laboratory investigation comparing Mayana leaf extracts prepared using 70% ethanol and 95% ethanol. The prepared stains were applied to urine sediment specimens and assessed based on selected staining criteria, including comparability to standard staining performance, stain stability, color enhancement, clarity of staining, cost-effectiveness, practicality, time efficiency, component differentiation, and recommendation potential. The findings showed that the 70% ethanol Mayana extract obtained a higher overall mean rating of 4.25 compared with the 95% ethanol extract, which obtained a mean rating of 2.25. The reported Mann-Whitney test result showed a p-value of 0.05, indicating a statistically significant difference between the two extract preparations at the 0.05 level of significance. The 70% ethanol extract performed better particularly in comparability to standard stains, stain stability, and color enhancement. The findings suggest that 70% ethanol may be a more suitable solvent concentration for preparing Mayana-based staining reagent for urine sediment observation. However, the study should be interpreted as a preliminary experimental evaluation, since full validation against Sternheimer-Malbin stain was not directly performed.
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Alshamar, H. A., & Dapson, R. W. (2021). Anthocyanins from a single botanical source can be used as a replacement for hemalum and eosin. Biotechnic & Histochemistry, 96(8), 570-578. https://doi.org/10.1080/10520295.2021.1966507
Bermido, C. M., Quinto, L. F., & Atento, R. G. O. (2025). A qualitative thematic review of contemporary challenges affecting health professions education: Implications for higher education leadership. International Journal of Health and Business Analytics, 1(2). https://doi.org/10.65166/yfm5w791
Diaz, L. K. R., Cheng, M. I. D., Espeja, R., Frayre, D. D. Q., Gammad, J. B., Hilario, J. A. B., Manalo, N. A. C., Santos, C. A. T., Tan, L. M. B., & Quinto, L. F. (2025). In-vitro anticoagulant and thrombolytic properties of crude Aspergillus terreus extract. International Journal of Health & Business Analytics, 1(2). https://doi.org/10.65166/60zfnq09
Japanese Association of Medical Technologists, Editorial Committee of the Special Issue: Urinary Sediment. (2017). Urinary sediment examination. Japanese Journal of Medical Technology, 66(J-STAGE-1), 51-85. https://doi.org/10.14932/jamt.17J12e
Kong, J. M., Chia, L. S., Goh, N. K., Chia, T. F., & Brouillard, R. (2003). Analysis and biological activities of anthocyanins. Phytochemistry, 64(5), 923-933. https://doi.org/10.1016/S0031-9422(03)00438-2
Laxamana, A. E., Ching, M. W., Tencomnao, T., Laxamana, M. P. N. O., & Prasanth, M. I. (2025). Anti-aging potential of crude methanolic extract from Cocos nucifera embryo using Caenorhabditis elegans as model organism. International Journal of Health & Business Analytics, 1(2), 1–15. https://doi.org/10.65166/azkk2x82
Maung Maung, Y., Myint Zu, K., & Than Win, T. (2018). Extraction and characterization of purple Coleus blumei natural dyes. International Journal of Innovations in Engineering and Technology, 9(4), 89-93. http://dx.doi.org/10.21172/ijiet.94.14
Morcilla, J. S., Al Bozom, I., Adham, A., Murshed, K., & Quinto, L. F. (2025). Investigation of an appropriate decalcifying agent for bone biopsy specimens submitted for histomorphologic analyses and immunohistochemical studies. International Journal of Health & Business Analytics, 1(2), 1–14. https://doi.org/10.65166/y1s38453
Nistor, M., Pop, R., Daescu, A., Pintea, A., Socaciu, C., & Rugina, D. (2022). Anthocyanins as key phytochemicals acting for the prevention of metabolic diseases: An overview. Molecules, 27(13), Article 4254. https://doi.org/10.3390/molecules27134254
Palaoro, L. A., & Angerosa, M. (2014). Correlation between the cytology of urine sediment in fresh sample and smears stained by Papanicolaou and Giemsa methods. Journal of Cytology, 31(1), 25-31. https://doi.org/10.4103/0970-9371.130666
Ylagan, H. M., Aguirre, K. A. G. C., Briones, L. A., Briones, P. N. P., Canapi, C. V. T., Corpuz, K. M. R., Esmelo, A. J. M., Ganacial, V. J. M., Gasid, K. M. M., Ledesma, A. F. P., Marasigan, K. V. O., Miraples, M. B., Santos, E. G. D., Valdez, J. F. L., & Quinto, L. F. (2025). Post-rehabilitation heavy metal contamination analysis of kangkong (Ipomoea aquatica) cultivated in the Pasig River Waterway, Manggahan Floodway. International Journal of Health & Business Analytics, 1(2). https://doi.org/10.65166/xf4mm102
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Copyright (c) 2026 Mary Jane E. Alba, Jeziel Khyl P. Ariamado, Mary Claire D. Busio, Angelito Miguel A. De Vera, Ross Alexandrei L. Fider, Janna Grace C. Gaa, Kristel Kaye A. Jiao (Author)
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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
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