Evidence of Anticoagulant Activity in Shallot (Allium ascalonicum) Extracts Based on Prothrombin and Activated Partial Thromboplastin Time Assays
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https://doi.org/10.65166/9c2w9464关键词:
Allium ascalonicum, shallot extract, anticoagulant activity, prothrombin time, activated partial thromboplastin time, in vitro, phytochemical screening摘要
Cardiovascular diseases remain a leading cause of mortality worldwide, with thrombosis as a major contributing factor, necessitating continued exploration of alternative and cost-effective anticoagulant agents. This study examined the anticoagulant activity of shallot (Allium ascalonicum) extracts using standard coagulation assays in human blood plasma. An experimental, quantitative design was employed using plasma obtained from fifteen healthy adult donors with blood type O. Aqueous shallot extracts were prepared at concentrations of 25%, 50%, and 75% and subjected to phytochemical screening. Anticoagulant activity was assessed using prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays, with untreated plasma and heparin-treated plasma serving as negative and positive controls, respectively. Phytochemical analysis confirmed the presence of flavonoids, alkaloids, saponins, glycosides, and tannins in the extract. One-way analysis of variance revealed significant differences in PT (p = 0.014) and aPTT (p < 0.001) values across treatment groups. Higher extract concentrations produced a dose-dependent prolongation of aPTT, while PT values showed significant but non-linear changes. The findings provide evidence that Allium ascalonicum extract demonstrates anticoagulant activity in human plasma under in vitro conditions, supporting further investigation of shallots as a potential natural anticoagulant source.
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